1. Introducció
This manual provides essential information for the proper use and handling of the Corning 3591 Polystyrene Flat Bottom 96 Well Not Treated EIA/RIA Clear Microplate. Adhering to these instructions will help ensure optimal performance and reliable experimental results.
2. Producte acabatview
The Corning 3591 microplate is a 96-well, flat-bottomed plate constructed from clear polystyrene. It features a 'not treated' (medium binding) surface, which is hydrophobic and facilitates biomolecule binding primarily through passive interactions. This design makes it suitable for applications requiring the immobilization of large molecules, such as antibodies, that possess significant hydrophobic regions.
Each microplate is individually wrapped and conforms to standard microplate footprint and dimensions. Lids are available separately.
Figura 1: An image showing the clear, flat-bottomed 96-well polystyrene microplate. The wells are arranged in an 8x12 grid, with alphanumeric labels along the edges for identification.
3. Configuració
The Corning 3591 microplate is supplied non-sterile. For applications requiring sterile conditions, appropriate sterilization procedures must be followed by the user, or a sterile product variant should be selected. Prior to use, ensure the microplate is at room temperature and free from any visible damage or contamination. Handle the microplate by the edges to avoid touching the well surfaces.
This microplate is designed to be compatible with standard laboratory equipment for 96-well plates, including plate readers, washers, and automated liquid handling systems.
4. Instruccions de funcionament
The 'not treated' polystyrene surface is hydrophobic and binds biomolecules through passive interactions. This surface is particularly effective for the immobilization of large molecules, such as antibodies, which have large hydrophobic regions capable of interacting with the plate surface.
- Recommended Working Volume: The recommended working volume for each well is 75 to 200 µL. The total well volume is 360 µL.
- Capacitat d'enquadernació: The approximate binding capacity for this surface is 250 ng of mouse IgG per square centimeter.
- Experimental Protocols: Follow established laboratory protocols for your specific EIA/RIA or other assays. Optimize reagent concentrations, incubation times, and washing steps according to your experimental requirements.
5. Manteniment
The Corning 3591 microplate is a single-use, disposable product. It is not intended for cleaning or reuse. Proper storage before use is crucial to maintain product integrity:
- Store in a clean, dry environment at room temperature.
- Keep the individually wrapped plates sealed until ready for use to prevent contamination.
Dispose of used microplates according to laboratory waste disposal guidelines and local regulations, especially if they contain biohazardous materials.
6. Solució De Problemes
Issues encountered during experiments using microplates are typically related to experimental design, reagent quality, or procedural errors rather than the physical integrity of the plate itself. If unexpected results occur, consider the following:
- Inconsistent Binding: Verify reagent concentrations, incubation temperatures, and times. Ensure proper mixing and consistent pipetting techniques.
- High Background: Optimize washing steps, blocking buffer composition, and antibody concentrations.
- Senyal baix: Check reagent activity, ensure proper coating conditions, and confirm detector functionality.
For specific concerns regarding the microplate's manufacturing quality, refer to the support section.
7. Especificacions
| Marca | Corning |
| Número de model | 3591 |
| Material | Poliestirè |
| Plate Format | 96-Doncs |
| Well Bottom | Pis |
| Well Shape | Ronda |
| Color de la placa | Clar |
| Tractament superficial | Not Treated (Medium Binding) |
| Total Well Volume | 360 µl |
| Recommended Working Volume | 75 a 200 µl |
| Binding Capacity (Mouse IgG) | Approx. 250 ng/sq cm |
| Esterilitat | No estèril |
| Embalatge | Individually Wrapped |
| Dimensions del paquet | 21.4 x 9 x 7.4 polzades |
| Pes del paquet | 7.35 Pounds (Case of 50) |
| ASIN | B005J0KPHE |
8. Informació de la garantia
Specific warranty details for the Corning 3591 microplate are not provided in the product information. For warranty inquiries, please contact Corning Life Sciences directly.
9. Suport
For technical assistance, product inquiries, or to request certification results for a specific lot, please contact Corning Life Sciences. Contact information can typically be found on the official Corning weblloc o embalatge del producte.
