1. Introducció
The AmScope D120A-MS is a dual-view compound monocular microscope designed for high magnification observation in biological and educational applications. It features a versatile viewing head, multiple objective lenses, Brightfield illumination, and a mechanical stage for precise specimen manipulation. This manual provides essential information for the proper setup, operation, and maintenance of your microscope.
2. Pautes de seguretat
- Manegeu sempre el microscopi amb cura. Eviteu moviments o impactes bruscos.
- Assegureu-vos que el microscopi estigui col·locat sobre una superfície estable i anivellada per evitar que es bolqui.
- Connect the power cord to a grounded electrical outlet of the correct voltage (110 V).
- No toqueu les superfícies òptiques amb les mans nues. Feu servir paper per a lents o un drap suau i sense borrissol per netejar-les.
- Unplug the microscope from the power source before cleaning or performing any maintenance.
- Mantingueu el microscopi allunyat de la llum solar directa, les altes temperatures, la pols i els productes químics corrosius.
- Supervise children or inexperienced users to ensure safe operation.
3. Contingut del paquet
Desembaleu amb cura tots els components i verifiqueu que hi hagi els elements següents:
- AmScope D120A-MS microscope with mechanical stage
- WF10x eyepiece
- WF16x eyepiece
- 4x DIN achromatic objective
- 10x DIN achromatic objective
- 40xS DIN achromatic objective (spring-loaded)
- 100xS (oil) DIN achromatic objective (spring-loaded)
- (2) Color filters
- Bombeta de recanvi
- Funda antipols
- Manual d'instruccions (aquest document)
4. Identificació de components
Familiarize yourself with the main parts of your AmScope D120A-MS microscope:
Figura 4.1: Acabatview of the AmScope D120A-MS microscope. This image displays the complete microscope assembly, including the base, stage, objective lenses, and dual-view monocular head.
- Eyepiece (Ocular): Where you look to view the specimen. The D120A-MS includes WF10x and WF16x eyepieces.
- Monocular ViewCap: The upper part of the microscope containing the eyepiece tube. This model features a dual-view head with a fixed 45-degree inclined tube and a vertical port.
- Rosca giratòria: Sosté les lents de l'objectiu i permet canviar fàcilment entre augments.
- Lents objectius: The primary magnification components (4x, 10x, 40xS, 100xS oil).
- Mecànica Stage: A platform where the specimen slide is placed, with controls for precise X-Y movement.
- Stage Clips: Secure the specimen slide on the mechanical stage.
- Botó d'enfocament aproximat: Gran botó per a ajustaments ràpids d'enfocament.
- Botó d'enfocament fi: Small knob for precise focusing adjustments.
- Il·luminador (font de llum): Situat sota el stage, provides light for viewing. This model uses variable-intensity tungsten illumination.
- Condensador Abbe: Located below the stage, focuses light onto the specimen. Features a 1.25 NA and an iris diaphragm.
- Diafragma d'iris: Controls the amount of light passing through the specimen.
- Base: The stable bottom support of the microscope.
5. Muntatge i muntatge
Follow these steps to set up your microscope for initial use:
- Desembalatge: Carefully remove the microscope and all accessories from the packaging. Retain the original packaging for future transport or storage.
- Col·locació: Place the microscope on a sturdy, level surface, away from vibrations and direct sunlight.
- Instal·lació de l'ocular: Insert the desired eyepiece (WF10x or WF16x) into the inclined eyepiece tube of the monocular head. Gently twist to secure it.
- Instal·lació objectiva: The objective lenses are typically pre-installed. If not, carefully screw each objective into the revolving nosepiece in increasing order of magnification (4x, 10x, 40xS, 100xS). Ensure they are finger-tight.
- Connexió d'alimentació: Connect the power cord to the microscope's power input and then to a standard 110V electrical outlet.
- Funda antipols: When not in use, cover the microscope with the provided dust cover to protect it from dust and debris.
Figura 5.1: Primer pla view of the objective lenses mounted on the revolving nosepiece. This image shows the 4x, 10x, 40xS, and 100xS (oil) objectives in position.
6. Funcionament bàsic
This section outlines the fundamental steps for observing specimens with your microscope.
- Encès: Flip the power switch located on the base of the microscope. The illuminator should light up.
- Ajustar la il·luminació: Use the variable intensity control knob to set the desired brightness. Adjust the iris diaphragm on the Abbe condenser to optimize contrast and resolution for your specimen.
- Col·locar la mostra: Rotate the revolving nosepiece to select the lowest power objective (4x). Lower the mechanical stage using the coarse focus knob. Place a prepared specimen slide onto the mechanical stage i assegura'l amb el stage clips.
- Position Specimen: Utilitzeu les XY stage control knobs to move the slide until the area of interest is centered under the objective lens.
- Enfocament:
- While looking through the eyepiece, slowly raise the stagi utilitzant el botó d'enfocament aproximat until the specimen comes into rough focus.
- Utilitza el botó d'enfocament fi to achieve a sharp, clear image.
- Canvia l'ampliació: Once focused at low power, rotate the revolving nosepiece to switch to a higher power objective (e.g., 10x, 40xS). Due to the parfocal design, only minor adjustments with the fine focus knob should be needed to bring the image back into sharp focus.
- Using the 100xS Oil Immersion Objective:
- After focusing with the 40xS objective, rotate the nosepiece halfway between the 40xS and 100xS objectives.
- Place a small drop of immersion oil directly onto the center of the specimen slide where the light passes through.
- Carefully rotate the 100xS objective into the oil drop until it clicks into place. The objective should be immersed in the oil.
- Utilitzeu només el fitxer botó d'enfocament fi to bring the image into sharp focus. Do not use the coarse focus knob with the 100xS objective.
- After use, clean the 100xS objective and the slide immediately with lens paper moistened with lens cleaning solution to remove all immersion oil.
- Dual-View Funcionalitat: The vertical port on the monocular head can accept a second eyepiece for simultaneous viewing by another person or a camera mount for digital image capture (eyepiece and camera sold separately). Dioptric adjustment on the upright tube accommodates individual eye-strength differences.
Figura 6.1: The AmScope D120A-MS microscope demonstrating its digital image capture capability by connecting to a laptop. This setup utilizes the vertical port for a camera attachment.
7. Manteniment i cura
Un manteniment adequat garanteix la longevitat i el rendiment òptim del vostre microscopi.
- Neteja de lents: Use only specialized lens paper and lens cleaning solution for optical surfaces. Gently wipe in a circular motion. Never use abrasive cloths or harsh chemicals.
- Neteja general: Netegeu el cos del microscopi amb un drap suau iamp drap. Eviteu que la humitat entri en els components elèctrics.
- Emmagatzematge: Always cover the microscope with the dust cover when not in use. Store it in a dry, dust-free environment.
- Substitució de bombetes: If the illuminator bulb burns out, unplug the microscope. Refer to the base of the microscope for access to the bulb compartment. Carefully replace the old bulb with the provided spare bulb, ensuring not to touch the glass part of the new bulb with bare hands.
- Parts mecàniques: Do not attempt to lubricate or disassemble any mechanical parts. If issues arise, contact customer support.
8. Solució De Problemes
Aquesta secció tracta problemes comuns que podeu trobar.
| Problema | Causa possible | Solució |
|---|---|---|
| Sense il·luminació | Power cord disconnected, power switch off, bulb burnt out | Check power connection, turn on switch, replace bulb |
| La imatge està borrosa o desenfocada | Incorrect focus adjustment, objective not fully engaged, dirty lens | Adjust coarse/fine focus, rotate nosepiece until objective clicks, clean lenses |
| Camp fosc de view | Iris diaphragm closed, illuminator too dim, condenser too low | Open iris diaphragm, increase illuminator brightness, adjust condenser height |
| Dust or spots in view | Dust on eyepiece, objective, or slide | Clean eyepiece, objective, or slide with lens paper |
| Cannot focus at 100x | No immersion oil, air bubbles in oil, coarse focus used | Apply immersion oil, remove air bubbles, use only fine focus |
9. Especificacions tècniques
Detailed specifications for the AmScope D120A-MS microscope:
- Cap: Compound dual-view monocular, 45-degree inclined, 360-degree rotatable
- Oculars: WF10x, WF16x (with pointer)
- Objectius: 4X, 10x, 40xS (spring), 100xS (oil, spring) DIN achromatic
- Interval d'ampliació: 40X - 1600X
- Stage: Mecànica stage with X-Y axis movement, stagi parada
- Configuració d'il·luminació: Transmitted (lower)
- Condensador: Abbe, 1.25 NA
- Diafragma: Diafragma de l'iris
- Font de llum: Tungsten (incandescent), variable intensity
- Tipus d'il·luminació: Camp clar
- Potència: 110 V
- Material: Metal, tungsten
- Dimensions (A x A x P): 15 x 7.5 x 5.125 polzades (aproximadament)
- Pes: 10 lliures
10. Garantia i atenció al client
For warranty information, technical assistance, or to purchase replacement parts, please contact AmScope customer support. Refer to the AmScope weblloc web o la documentació de la compra per obtenir les dades de contacte actuals.
