Introducció
The Millipore Amicon Ultra Centrifugal Filters, model UFC803024, are advanced tools designed for efficient protein concentration and desalting. These units are supplied pre-assembled with centrifuge tubes and caps, featuring a low-binding regenerated cellulose membrane for fast processing and high recovery rates, typically exceeding 90 percent. The unique vertical housing design optimizes performance for various laboratory applications.
Typical applications include the purification of macromolecular components from tissue culture extracts or cell lysates, as well as the concentration of biological samples. This manual provides essential information for the proper setup, operation, maintenance, and troubleshooting of your Amicon Ultra Centrifugal Filters.
Producte acabatview
This image displays the Amicon Ultra Centrifugal Filter unit. It consists of a main clear centrifuge tube with volume markings and a black screw cap, designed for sample processing. Below it, a smaller, clear collection tube is shown, which fits into the larger unit to collect the filtered sample.
The Amicon Ultra Centrifugal Filter unit is comprised of a filter device with a regenerated cellulose membrane and a collection tube. The membrane's low-binding properties ensure minimal sample loss, while the vertical design facilitates efficient filtration. Each package contains 24 units, ready for immediate use.
Configuració
- Desembalatge i inspecció: Carefully remove the Amicon Ultra Centrifugal Filters from their packaging. Inspect each unit for any signs of damage during transit. Do not use damaged units.
- Preparació: Ensure your centrifuge is clean and in good working order. Verify that the centrifuge rotor is compatible with the dimensions of the Amicon Ultra units (typically 15 mL conical tube rotors).
- Pre-wetting (Optional, but Recommended for some applications): For optimal performance and to minimize non-specific binding, some protocols recommend pre-wetting the membrane with a small volume of buffer (e.g., 0.5 mL of deionized water or appropriate buffer) and centrifuging briefly to remove the buffer. Discard the flow-through.
Instruccions de funcionament
Follow these steps for effective use of the Amicon Ultra Centrifugal Filters:
- Sample Loading: Pipette your sample into the upper reservoir of the Amicon Ultra filter unit. Ensure the sample volume does not exceed the maximum capacity (4 mL for this model).
- Centrifugació: Place the loaded filter unit into a compatible centrifuge rotor. Ensure the rotor is balanced by placing an identical, balanced unit (e.g., another loaded filter or a tube with an equivalent volume of water) opposite your sample. Centrifuge at the recommended speed and time for your specific application. Refer to your protocol for exact parameters (e.g., 3,000-5,000 x g for 10-30 minutes).
- Sample Recovery: After centrifugation, carefully remove the filter unit from the centrifuge. Separate the filter device from the collection tube. The concentrated sample will be retained in the upper reservoir of the filter device. The filtrate (unwanted components) will be in the collection tube.
- Retrieving Concentrated SampLI: Invert the filter device into a clean, new collection tube (or the original collection tube if the filtrate is not needed). Centrifuge briefly (e.g., 1,000 x g for 2 minutes) to recover the concentrated sample from the membrane.
- Desalting/Buffer Exchange (if applicable): For desalting or buffer exchange, add an appropriate volume of the desired buffer to the concentrated sample in the filter device and repeat the centrifugation step. This process can be repeated multiple times to achieve the desired buffer exchange.
Consideracions importants:
- Always balance your centrifuge rotor.
- Do not exceed the maximum recommended centrifugal force (RCF) for the device.
- Ensure the temperature during centrifugation is appropriate for your sample.
Manteniment
Amicon Ultra Centrifugal Filters are designed for single-use applications. They are not intended for cleaning or reuse. Proper maintenance primarily involves correct handling and disposal:
- Manipulació: Always handle the units carefully to avoid damaging the membrane or the plastic housing.
- Eliminació: After use, dispose of the filter units and any associated waste according to your laboratory's standard operating procedures for biological or chemical waste, and local regulations.
Resolució de problemes
If you encounter issues during the use of your Amicon Ultra Centrifugal Filters, consider the following:
- S baixample Recovery:
- Causa: Membrane clogging, sample adherence to the membrane, or incorrect recovery technique.
- Solució: Ensure proper pre-wetting if recommended for your sample type. Optimize centrifugation speed and time. Ensure complete recovery by inverting and centrifuging the device into a new collection tube.
- Slow Filtration Rate:
- Causa: Alt sample viscosity, particulate matter in the sample, or membrane clogging.
- Solució: Pre-clarify samples with high particulate loads (e.g., by centrifugation or pre-filtration). Adjust centrifugation speed if possible, within device limits.
- Leakage from Unit:
- Causa: Damaged unit, improper assembly, or exceeding maximum sampel volum.
- Solució: Inspect units before use. Ensure the cap is securely fastened. Do not overfill the reservoir.
- Membrane Damage:
- Causa: Physical abrasion, chemical incompatibility, or excessive centrifugal force.
- Solució: Handle units gently. Ensure sample components are compatible with regenerated cellulose. Do not exceed recommended RCF.
Especificacions
- Número de model: UFC803024
- Membrane Material: Regenerated Cellulose (Ultracel®)
- Nominal Molecular Weight Limit (NMWL): 30 kDa
- S màximampel volum: 4 ml
- Final Concentrate Volume: Typically 20-100 µL
- Typical Recovery: >90%
- Quantitat del paquet: 24 unitats
- Dimensions del paquet: 5.98 x 5.63 x 4.21 polzades
- Pes del paquet: 10.4 unces
- País d'origen: Irlanda
Garantia i Suport
Specific warranty information for the Millipore Amicon Ultra Centrifugal Filters UFC803024 should be obtained directly from the manufacturer, Millipore, or your authorized distributor at the time of purchase. Generally, products are warranted against defects in materials and workmanship under normal use.
For technical support, product inquiries, or to report any issues, please contact your supplier or the manufacturer's customer service department. Have your product model number (UFC803024) and lot number ready when contacting support.
